Terminator

Part:BBa_K2215005:Design

Designed by: Megan Sare   Group: iGEM17_ULaVerne_Collab   (2017-09-11)


Putative Symbiodinium Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6
    Illegal suffix found in sequence at 328
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal SpeI site found at 329
    Illegal PstI site found at 343
    Illegal NotI site found at 12
    Illegal NotI site found at 336
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6
    Illegal suffix found in sequence at 329
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6
    Illegal XbaI site found at 21
    Illegal SpeI site found at 329
    Illegal PstI site found at 343
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Determined the sequence from Symbiodinium genome by counting about 300 bp after the stop codon.


Source

Levin, Rachel et al., (2017). "Engineering Strategies to Decode and Enhance the Genomes of Coral Symbionts."

References